This course is suitable for analysts looking to explore different chromatographic techniques/modes applicable for the analysis of polar molecules. It will introduce you to a number of possible techniques that can be employed to retain polar molecules, generally hard to retain using C18 columns in reversed-phase solvent systems. It includes details of new column chemistries suitable for reversed-phase retention of polars, such as polar embedded columns, as well as alternative techniques including: ion pairing, mixed-mode, Hydrophilic Interaction Liquid Chromatography (HILIC), Aqueous Normal Phase (ANP) and Supercritical Fluid Chromatography (SFC). This course allows you to explore the various options available to expand the polarity range of molecules that can be retained and discusses how these complement standard reversed-phase methodology.
Limitations of reversed-phase HPLC
Alternatives to C18
Ion pairing
HILIC
Aqueous normal-phase
SFC
Available as in-house or off-site courses. Contact us for details
Limitations of reversed-phase HPLC
Alternatives to C18
Ion pairing
HILIC
Aqueous normal-phase
SFC
Available as in-house or off-site courses. Contact us for details
