This course provide a complete training solution enabling you to understand your Liquid Chromatograph or LC-MS instrument, application method development, troubleshooting & maintenance.
This course covers all the practical theory you need to know about liquid chromatography and its related techniques including liquid chromatography-mass spectrometry. Mobile phase optimisation including gradient elution, pumps and plumbing, autosampler injection techniques, sample preparation, calibration standards, data analysis for quantitation and qualitative analysis are discussed.
It also looks at how to develop appropriate application specific HPLC & LC-MS methods and the set-up and optimisation of instrumentation. Finally the steps required to maintain and troubleshoot the system, problem chromatography and mass spectra are covered. Advanced techniques are discussed and their application to dificult samples requiring optimal limits of detection and linear dynamic range. HPLC to UHPLC method transfer is touched upon, chromatographic deconvolution utilising tandem mass spectrometry, multi-dimensional LC (LCxLC), on-line SPE and chemometrics.
This course covers all the practical theory you need to know about liquid chromatography and its related techniques including liquid chromatography-mass spectrometry. Mobile phase optimisation including gradient elution, pumps and plumbing, autosampler injection techniques, sample preparation, calibration standards, data analysis for quantitation and qualitative analysis are discussed.
It also looks at how to develop appropriate application specific HPLC & LC-MS methods and the set-up and optimisation of instrumentation. Finally the steps required to maintain and troubleshoot the system, problem chromatography and mass spectra are covered. Advanced techniques are discussed and their application to dificult samples requiring optimal limits of detection and linear dynamic range. HPLC to UHPLC method transfer is touched upon, chromatographic deconvolution utilising tandem mass spectrometry, multi-dimensional LC (LCxLC), on-line SPE and chemometrics.
