Please join us for the June edition of the PPSG Early Career Academic seminar series. This month's speakers will be Dr Guto Rhys from Cardiff University and Dr Andrew Brennan from the University of Bath
Free online Zoom lecture
Registration: https://eu01web.zoom.us/meeting/register/u5Apcemqqz0iHNCpxwFSI6FklH8onAKQ1FpB
Guto Rhys 鈥� Cardiff University
Title: Exploring new folds and functions based on polypeptides
Abstract: In this talk I will describe my research journey in discovering, rationalising, and designing new protein folds based on a class of associating peptides called coiled coils. I will highlight how these new-to-nature structures can be applied to novel applications. Finally, I will briefly discuss recently establishing the Rhys Lab at Cardiff University where we are beginning to engineer and design artificial metalloenzymes.
Andrew Brennan - University of Bath
Title: Transcription Block Survival: An Intracellular Peptide Library Screen to Identify Potent Antagonists
Abstract: Transcription Block Survival (TBS) is a high-throughput, intracellular peptide screening platform to derive functional transcription factor antagonists. TBS is an entirely tag-free genotype-to-phenotype approach, selecting for desirable attributes such as high solubility, target specificity and low toxicity within a complex cellular environment. The assay has been validated against a number of transcriptional regulators including cJun, which is overactive in a range of diseases. In vivo library screening against cJun has identified a sequence that both binds cJun and antagonises function, which has been subsequently optimised through rational design.
If you would like to present in future seminars, please contact one of the organisers. We welcome presentations from early career UK-based academics or senior postdoctoral researchers seeking to establish an independent career in peptide and protein science.
Louis Luk: lukly@cardiff.ac.uk
Chris Coxon: chris.coxon@ed.ac.uk
Louise Walport: louise.walport@crick.ac.uk
Free online Zoom lecture
Registration: https://eu01web.zoom.us/meeting/register/u5Apcemqqz0iHNCpxwFSI6FklH8onAKQ1FpB
Guto Rhys 鈥� Cardiff University
Title: Exploring new folds and functions based on polypeptides
Abstract: In this talk I will describe my research journey in discovering, rationalising, and designing new protein folds based on a class of associating peptides called coiled coils. I will highlight how these new-to-nature structures can be applied to novel applications. Finally, I will briefly discuss recently establishing the Rhys Lab at Cardiff University where we are beginning to engineer and design artificial metalloenzymes.
Andrew Brennan - University of Bath
Title: Transcription Block Survival: An Intracellular Peptide Library Screen to Identify Potent Antagonists
Abstract: Transcription Block Survival (TBS) is a high-throughput, intracellular peptide screening platform to derive functional transcription factor antagonists. TBS is an entirely tag-free genotype-to-phenotype approach, selecting for desirable attributes such as high solubility, target specificity and low toxicity within a complex cellular environment. The assay has been validated against a number of transcriptional regulators including cJun, which is overactive in a range of diseases. In vivo library screening against cJun has identified a sequence that both binds cJun and antagonises function, which has been subsequently optimised through rational design.
If you would like to present in future seminars, please contact one of the organisers. We welcome presentations from early career UK-based academics or senior postdoctoral researchers seeking to establish an independent career in peptide and protein science.
Louis Luk: lukly@cardiff.ac.uk
Chris Coxon: chris.coxon@ed.ac.uk
Louise Walport: louise.walport@crick.ac.uk
